Let’s learn something about TLC( The layer chromatography) ?

The layer chromatography (TLC) is a method by which stationary phase is uniformly coated on a glass, plastic or metal plate with a smooth surface to form a thin layer for chromatographic separation. It belongs to plate chromatography and is a commonly used chromatographic separation method

TLC method is used by many national pharmacopoeia for the examination of impurities in drugs, drug analysis and other aspects, and is currently one of the most collected in the pharmacopoeia of the identification and detection of related substances, with simple equipment, simple operation, fast separation speed, sensitivity and high resolution

Usually,if your material is same with stationary phase,and it has the same Chromatography,then it can be regards , your product is pass the ID

For the ID you didn’t pass , it has following reasons

Methodology

Different detection institutions have different methods. ID cannot be confirmed without clear detection method

After the method is confirmed, the different reference products will affect the results, especially when using plant raw materials to be the standard substance ,the origin growth environment,Climate ,temperature has very import effect for the raw material

Extract solvents

Alcohol extraction can be used to extract alcohol-soluble components. Water extraction can be used to extract water extract. Some elements extracted from alcohol as standard may not be used to extract water.so it can make your ID didn’t pass

Extraction proportion

If extraction proportion is too low, it maybe can’t pass ID and even the extraction proportion is high , it still can’t sure to pass the ID. some of the pure powder without excipients still can't pass ID ,

The comprehensive influence factors of raw material by origin place ,detection methods, reference standard and solvents